DNA

Part:BBa_K5276015

Designed by: Yuming Luo   Group: iGEM24_UCAS-China   (2024-09-26)


Bxb1B-GT

This is a specific recognition site for recombinase Bxb1. It pairs with Bxb1P-GT (BBa_K5276016). Bxb1 can catalyze inversion when the sites are anti-aligned or excision when the sites are aligned. For a detailed mechanism, please refer to the part of recombinase Bxb1(BBa_K5276002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23


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Categories
Parameters
None